Biotinylated Human STEAP2 Full Length Protein, Flag,Avitag™ (Nanodisc-pro)

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Product Details

  • Synonym

    STEAP2

  • Source

    Biotinylated Human STEAP2 Full Length Protein, Flag,Avitag (ST2-H85D3) is expressed from Baculovirus-Insect cells. It contains AA Glu 2 - Met 490 (Accession # Q8NFT2-1).

    Predicted N-terminus: Met

    Request for sequence
  • Molecular Characterization

    STEAP2 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a flag tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 59.7 kDa.

    Nanodiscs are a new class of model membranes that are being used to solubilize and study a range of integral membrane proteins and membrane-associated proteins. The Nanodisc bilayer is bounded by an amphipathic scaffold coat that confers enhanced stability and a narrow particle size distribution.

    The nanodisc-pro is directly extracted from cell membrane in a water-soluble form using an amphipathic scaffold followed by affinity chromatography, and thus, it conserves a patch of native membrane around them.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process
  • *The isotype control of empty/mock nanodisc (Cat. No. APO-H5143) is sold separately and not included in protein, you can follow the link for product information.

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Performance Data

  • Bioactivity-ELISA

     STEAP2 ELISA

    Immobilized Biotinylated Human STEAP2 Full Length Protein, Flag,Avitag (Cat. No. ST2-H85D3) at 5 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-STEAP2 Antibody with a linear range of 0.04-1.25 μg/mL (QC tested).

    Protocol

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Background

This gene is a member of the STEAP family and encodes a multi-pass membrane protein that localizes to the Golgi complex, the plasma membrane, and the vesicular tubular structures in the cytosol. A highly similar protein in mouse has both ferrireductase and cupric reductase activity, and stimulates the cellular uptake of both iron and copper in vitro. Increased transcriptional expression of the human gene is associated with prostate cancer progression. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

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