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Product Specifications
Assay TypeSandwich-ELISAAnalyteCapsid protein p24Format96T (8×12 strips)ReactivityHIV (HV1-HXB2)Regulatory StatusRUOSensitivity<7.81 pg/mLStandard Curve Range250-7.81pg/mLAssay Time3 hr 20 minSuitable Sample TypeFor the quantitative determination of HIV-1 p24 in Cell Culture Supernatants, Plasma, Serum.Sample volume100 μLProduct Overview
HIV-1 p24 ELISA Kit (Residue Testing) is based on the ELISA sandwich method and is used for the quantitative detection of Human Immunodeficiency Virus-1 p24 (HIV-1 p24) in samples from CAR-T product preparation processes where it is used as a lentivirus vector. It is designed to provide a reliable solution for CAR-T product quality assessment during drug development and CMC quality control stages.
Reconstitution
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeRES050-C01Pre-coated Anti-HIV-1 p24 Antibody Microplate1 plate (8×12 strips)RES050-C02HIV-1 p24 Standard20 μgRES050-C03Biotin-Anti-HIV-1 p24 Antibody20 μgRES050-C04Streptavidin-HRP50 μLRES050-C0510xWashing Buffer50 mLRES050-C062xDilution Buffer50 mLRES050-C07Substrate Solution12 mLRES050-C08Stop Solution7 mLRES050-C091xLysis Buffer12 mLACRO Quality Management System
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Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
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