Rhesus macaque / Cynomolgus Complement Factor D / CFD Protein, His Tag (active enzyme)

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Cat. No. / Size
Price
Qty
CFD-R52H3-100ug
₩551,000
CFD-R52H3-1mg (250ug X 4)
₩4,000,000
ETA of in-stock products:2 business days

Product Details

  • Synonym

    CFD, Adipsin, PFD, DF, Complement factor D

  • Source

    Rhesus macaque / Cynomolgus Complement Factor D, His Tag (CFD-R52H3) is expressed from human 293 cells (HEK293). It contains AA Ile 26 - Ala 253 (Accession # H9EXC1-1). In the region Ile 26 - Ala 253, the AA sequence of Rhesus macaque and Cynomolgus Complement Factor D are homologus.

    Predicted N-terminus: Ile 26

    Request for sequence

  • Molecular Characterization

    Complement Factor D Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 26.5 kDa. The protein migrates as 25-27 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Complement Factor D SDS-PAGE

    Rhesus macaque / Cynomolgus Complement Factor D, His Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • Bioactivity

    Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is >40 pmol/min/μg (QC tested).

    Protocol

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Background

Complement factor D (CFD) is also known as Adipsin, C3 convertase activator, Properdin factor D (PFD), which contains one peptidase S1 domain and belongs to the peptidase S1 family. CFD / Adipsin cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. CFD / Adipsin is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis. Defects in CFD / Adipsin are the cause of complement factor D deficiency which predisposes to invasive meningococcal disease.

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Drug Development Progress
  • English Name:

    Complement factor D

  • Category:

  • Approved Drugs:

    1 Details

  • Drugs in Clinical Trials:

    5 Details

  • Highest Development Stage:

    Approved

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