Product Details
Product Details
This antibody panel comprises two distinct rabbit monoclonal antibodies against Pyrrolobenzodiazepine (PBD), with slight differences in their recognition epitopes. Consequently, they may exhibit varying affinity levels towards different PBD analogs or different conjugates of PBD and linkers. This panel can be used to conduct preliminary experiments for screening the optimal antibody clone.
Components
Cat.No.CloneSizePAD-MY2221M1D0820 ugPAD-MY22121M1F920 ugSpecificity
Specifically recognizes PBD.
Source
This panel contains Two anti-PBD monoclonal antibodies, suitable for pharmacokinetic (PK) detection of ADC with PBD as the payload. All the anti-PBD monoclonal antibodies are recombinantly expressed from HEK293, can be used as a capture antibody for detecting conjugated antibodies in ELISA assay.
Isotype
Rabbit IgG | Rabbit Kappa
Purification
Protein A purified / Protein G purified
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
Bioactivity-ELISA

Immobilized Monoclonal Anti-Payload PBD Antibody, Rabbit IgG (M1D08) (Cat. No. PAD-MY2221) at 1 μg/mL, add Loncastuximab Tesirine in the 100% Human Serum and then add Biotinylated Human CD19 (20-291), His,Avitag, premium grade (Cat. No. CD9-H82E9) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (QC tested).
Protocol
Immobilized Monoclonal Anti-Payload PBD Antibody, Rabbit IgG (1M1F9) (Cat. No. PAD-MY2212) at 1 μg/mL, add Loncastuximab Tesirine in the 100% Human Serum and then add Biotinylated Human CD19 (20-291), His,Avitag, premium grade (Cat. No. CD9-H82E9) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (QC tested).
Protocol
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