Human TL1A / DcR3 Inhibitor Screening Kit (TR-FRET)

FAB

1. Comprehensive validation - Validated with various antibody drugs.
2. Simple and fast operation - No complicated washing steps, significantly reducing time.
3. High batch consistency - Strict control over raw materials and finished product quality, ensuring a stable supply.
4. Accurate and reliable results - High sensitivity with minimal matrix effects.
5. High throughput capability - Support multiple product specifications, ideal for high-throughput screening.
6. Fast completion - Results in just 1 hour.

Product Specifications

Product Overview

The Human TL1A / DcR3 Inhibitor Screening Kit (TR-FRET) is based on a homogeneous (no wash) competitive TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer) to screen for inhibitors of human TL1A binding to DcR3 within 0.5-1 hours. It can also be used as a universal detection tool to identify the ability of human TL1A to bind to human DcR3.

Storage

1. Store the unopened kit at 2-8°C upon receipt.

2. Locate the expiration date on the outer packaging and do not use reagents beyond their expiration date.

3. The opened kit should be stored according to the storage conditions listed in the Components Table. The shelf life of the opened kit is 30 days from the date of opening.

Materials Provided

Assay Principles

This Human TL1A / DcR3 Inhibitor Screening Kit (TR-FRET) is based on TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer). This assay uses the mixture of biotinylated human TL1A and Europium-chelate labeled streptavidin as the Donor and FA Labeled Human DcR3 Protein as the Acceptor.

— When the sample does not contain the inhibitors that block the binding of human TL1A to human DcR3, the Donor and Acceptor are in close proximity because of the binding of human TL1A and human DcR3. Upon Donor excitation with light of a specific wavelength (337nm), in addition to Donor emission (620nm), non-radiative transfer of energy occurs between Donor and Acceptor, resulting in Acceptor emission (665nm).

— When the sample contains the inhibitors that block the binding of human TL1A to human DcR3, the components inhibit the binding between the Donor and Acceptor and thereby prevent FRET from occurring.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process