Human IFN-gamma Detection Kit (TR-FRET)

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Product Details

  • Product Overview

    Human IFN-gamma Detection kit (TR-FRET) can detect the human IFN-gamma in homogeneous system within 2 hours, it is highly sensitive, short detection time, easy to use and possesses high-throughput screening capability. Compatible with microplate formats, this kit supports high-throughput screening workflows, enabling efficient large-scale sample analysis. This kit is specifically designed for the accurate quantitation of human IFN-gamma from cell culture supernatant, serum and plasma.

    1. Simplified and straightforward protocols and ready-to-use reagents to save assay time.
    2. Standards to calibrate with NIBSC/WHO standards for comparable results.
    3. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
    4. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.

  • Product Specifications

    Assay Type
    Sandwich-TR-FRET
    Analyte
    Human IFN-gamma
    Format
    100T/500T
    Reactivity
    Human
    Sensitivity
    LOQ=46.64pg/mL
    Assay Time
    2 hr
    Sample volume
    16 μL
    Range
    46.64 pg/mL-4000 pg/mL
    Sample Type
    For human TNF-α detection in human serum, human plasma,cell culture supernatants.

    Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

  • Application

    The kit is developed for quantitative detection of human IFN-γ in serum and cell culture supernates.

    It is for research use only.

  • Workflow

    IFN-γ Workflow

  • Storage

    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table.

  • Materials Provided

    ID
    Components
    Size
    FRTC01-C01
    Anti-Human IFN-γ Antibody Europium-chelate
    100 tests/500 tests
    FRTC01-C02
    FA Labeled Anti-Human IFN-γ Antibody
    100 tests/500 tests
    FRTC01-C03
    Human IFN-γ Standard
    20 μg/100 tests & 500 tests
    DB-04
    TR-FRET Sample Dilution Buffer, pH7.4
    50 mL/100 tests & 500 tests
    DB-05
    TR-FRET Detection Buffer, pH7.4
    50 mL/100 tests & 500 tests
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Typical Data 

    Please refer to DS document for the assay protocol.

     IFN-γ TYPICAL DATA

    Standard curve generated in TR-FRET Sample Dilution Buffer, pH7.4 (DB-04).

Validation
  • Dilution Linearity

    To evaluate the linearity of the assay, samples spiked with high-concentration human IFN-gamma in various matrices were prepared. With DB-04 (TR-FRET Sample Dilution Buffer, pH 7.4) as the surrogate matrix, standard curves were generated and the spiked samples were subjected to serial gradient dilution, followed by the quantification of the spiked samples.

     IFN-γ DILUTION LINEARITY
  • Intra-Assay Statistics

    Five samples with known concentrations were tested repeatedly 16 times within a single analytical batch to assess within-batch precision and accuracy.

     IFN-γ INTRA-ASSAY STATISTICS
  • Inter-Assay Statistics

    Five samples with known concentrations were tested repeatedly across three independent analytical batches to evaluate between-batch precision and accuracy.

     IFN-γ INTER-ASSAY STATISTICS

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