Save ProductProduct Details
Source
Monoclonal Anti-C11D5.3 scFv Antibody, Mouse IgG1 (3G8C1) is a non-neutralizing antibody expressed from human HEK293 cells, which provides higher batch consistency and long term security of supply.
Application
Flow Cytometry (Evaluation of Anti-BCMA (C11D5.3 scFv) CAR Expression).
Clone
3G8C1
Species
Mouse
Isotype
Mouse IgG1 | Mouse kappa
Specificity
Specifically recognizes C11D5.3 derived CARs.
Immunogen
Recombinant C11D5.3 scFv derived from HEK293 cell
Conjugate
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
Isotype Control
The isotype control is sold separately; please refer to Cat. No. DNP-PM1 for product information.
Recommended Dilution
1:50
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300 and 0.2% BSA, with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
For best performance, we strongly recommend following the reconstitution protocol provided in the CoA.
Storage
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months after reconstitution.
- 2-8 °C for 12 months after reconstitution.
ACRO Quality Management System
Customers Also Viewed
Performance Data
Bioactivity-FACS
Flow cytometric analysis of Anti-BCMA(C11D5.3) CAR-293 cells staining with PE-Labeled Monoclonal Anti-C11D5.3 scFv Antibody, Mouse IgG1 (3G8C1) (Cat. No. C13-PCFMY2) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with Isotype control antibody. PE signal was used to evaluate the binding activity (QC tested).
Protocol
Non-specificity of PE-Labeled Monoclonal Anti-C11D5.3 scFv Antibody, Mouse IgG1 (3G8C1) (Cat. No. C13-PCFMY2) binding to CD3+ cells present in human PBMC. 5e5 of human PBMCs were simultaneously stained with FITC-Labeled Monoclonal Anti-Human CD3 Antibody and PE-Labeled Monoclonal Anti-C11D5.3 scFv Antibody, Mouse IgG1 (3G8C1) (2 μL of the antibody stock solution corresponds to labeling of 5e5 cells in a final volume of 100 µL) and washed and then analyzed with FACS. Both FITC and PE positive signals was used to evaluate the non-specific binding activity to human CD3+ cells (QC tested).
Protocol
Compared Data
Flow cytometric analysis of Anti-BCMA (C11D5.3) CAR-293 cells staining with PE-Labeled Monoclonal Anti-C11D5.3 scFv Antibodies. PE signal was used to evaluate the binding activity of anti-C11D5.3 scFv antibody. The biological activity level of C13-PCFMY2 is superior to Company B (Routinely tested).
Protocol
Customer Reviews Writing Reviews
Background
Recent Advances
Contact Us
전화
글로벌: +1 800-810-0816 (무료)
아시아태평양: +86 400-682-2521
Drug Candidate Licensing
