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PE-Labeled Human HLA-A*03:01&B2M Tetramer Protein (Peptide free)

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Qty.
HLM-HP2H6-25ug
¥4160.00
HLM-HP2H6-200ug
¥23940.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    HLA-A*0301 & B2M

  • 表达区间及表达系统(Source)

    PE-Labeled Human HLA-A*03:01&B2M Tetramer Protein (HLM-HP2H6) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*03:01) & Ile 21 - Met 119 (B2M) (Accession # P04439-1 (HLA-A*03:01) & P61769 (B2M)).

    Predicted N-terminus: Gly 25 & Ile 21

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    PE-Labeled Human HLA-A*03:01&B2M Tetramer Protein is assembled by biotinylated monomer (HLM-H82Ey) and PE-labeled streptavidin.

    Biotinylated Human HLA-A*03:01&B2M Monomer Protein is produced by co-expression of HLA and B2M. This Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

  • 偶联(Conjugate)

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, 1% BSA, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

数据展示

  • 活性(Bioactivity)-ELISA

     HLA-A*0301 & B2M ELISA

    Immobilized PE-Labeled Human HLA-A*03:01&B2M Tetramer Protein (Cat. No. HLM-HP2H6) at 1 μg/mL (100 μL/well) can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.06-4 ng/mL (QC tested).

    Protocol
  •  HLA-A*0301 & B2M ELISA

    Immobilized PE-Labeled Human HLA-A*03:01&B2M Tetramer Protein (Cat. No. HLM-HP2H6) at 1 μg/mL (100 μL/well) can bind Anti-B2M Antibody, Human IgG1 with a linear range of 0.06-2 ng/mL (Routinely tested).

    Protocol

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背景介绍

Major histocompatibility complex (MHC), group of genes that code for proteins found on the surfaces of cells that help the immune system recognize foreign substances. MHC proteins are found in all higher vertebrates. In human beings the complex is also called the human leukocyte antigen (HLA) system.

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