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- ProductSizeAmountActiveMax® Human BCMA μBeads, premium grade (for cells)2.5 mg2.5×10⁷ beadsActiveMax® Human BCMA μBeads, premium grade (for cells)10 mg (2.5 mg×4)1.0×10⁸ beads
产品描述（Product Description）
ActiveMax® Human BCMA μBeads, premium grade (for cells) are uniform, superparamagnetic beads of 5.5 µm in diameter immobilized with Human BCMA protein expressed from human 293 cells (HEK293) and contains AA Met 1 - Ala 54 (Accession # Q02223-1).
ActiveMax® Human BCMA μBeads, premium grade (for cells) are produced under sterile manufacturing conditions (ISO 5), and no animal- or human-derived components are used throughout the production process. It is produced under our rigorous quality control system that includes a comprehensive set of tests including sterility and endotoxin tests.应用说明（Application）
ActiveMax® Human BCMA μBeads, premium grade (for cells) are designed to stimulate in vitro BCMA-specific CAR-T cells or UCAR-T cells, similar to the tumor cell lines that express human BCMA antigen. It can be used as follows:
Evaluating the characteristics of CAR-T cells or UCAR-T cells.
In vitro expansion of BCMA-specific CAR-T cells or UCAR-T cells.
In vitro enrichment of BCMA-specific CAR-T cells or UCAR-T cells.重构方法（Reconstitution）
See Certificate of Analysis (CoA) for detailed instruction.
存储（Storage）
This product is stable in storage under the following conditions: -20˚C for 12 months in lyophilized state. -70°C for 3 months under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles after reconstitution. Immediate use after reconstitution is highly recommended.
无菌（Sterility）
Negative
内毒素（Endotoxin）
Less than 0.002 EU per μg by the LAL method / rFC method.
注意事项（Important Note）
This product is for research use only and not intended for therapeutic or in vivo diagnostic use.
制剂（Formulation）
Please contact us for detailed information.
Contact us for customized product form or formulation.
质量管理控制体系（QMS）
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数据展示
流式数据 -FACS Data
Assay of human BCMA protein on the μBeads surface by Flow cytomtry. The human BCMA conjugated on the μBeads (Cat. No. MBS-C004) surface were fluorescently stained using PE labeled anti-human BCMA antibody and analyzed by flow cytometry.
Protocol
批间一致性（Batch Consistency）
Add 100ul of 1:40 PE anti-human BCMA Antibody dilution (2.5ul stock solution in 100ul FACS buffer) into 5e5 of ActiveMax® human BCMA μBeads, premium grade (for cells) (Cat. No. MBS-C004), negative control beads (ActiveMax® Streptavidin μBeads, premium grade (for cells) (Cat. No. MBS-C009)) as well. PE signals was used to evaluate BCMA beads binding activity.
稳定性（Stability）
Add 100ul of 1:40 PE anti-human BCMA Antibody dilution (5ul stock solution in 100ul FACS buffer) into 5e5 of ActiveMax® human BCMA μBeads, premium grade (for cells) (Cat. No. MBS-C004), Negative control (ActiveMax® Streptavidin μBeads, premium grade (for cells) (Cat. No. MBS-C009)) as well. PE signals was used to evaluate BCMA beads binding activity.
刺激BCMA CAR-T细胞 -Cytokines
ActiveMax® Human BCMA μBeads, premium grade (for cells) (Cat.No.MBS-C004) can activate BCMA-specific CAR-T cells by detecting the secretion of IFN- γ in vitro.
BCMA CAR-T cells were stimulated with ActiveMax® Human BCMA μBeads (MBS-C004) for 24 h , and cell-free supernatants were harvested for evaluating IFN-γ、TNF-α、IL-4、IL-6 secretion by flow cytomtry. The results showed that BCMA CAR-T cells released significantly larger amounts of IFN-γ、TNF-α、IL-4、IL-6 into the supernatants in response to BCMA μBeads.
刺激BCMA CAR-T细胞 -Cytotoxic Molecules
BCMA CAR-T cells were stimulated with ActiveMax® Human BCMA μBeads (MBS-C004) for 24h, and cell-free supernatants were harvested for evaluating Granzyme B, Perforin secretion by ELISA. The results showed that BCMA CAR-T cells released significantly larger amounts of Granzyme B, Perforin into the supernatants in response to BCMA μBeads.
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