产品详情
分子别名(Synonym)
SHPS1,SIRPA,CD172A,BIT,MFR,MYD1,P84,PTPNS1
表达区间及表达系统(Source)
Biotinylated Mouse SIRP alpha Protein, Fc,Avitag (SIA-M82F7) is expressed from human 293 cells (HEK293). It contains AA Lys 32 - Asn 373 (Accession # P97797-1).
Predicted N-terminus: Lys 32
Request for sequence蛋白结构(Molecular Characterization)
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 66.0 kDa. The protein migrates as 90-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记(Labeling)
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度(Protein Ratio)
Passed as determined by the HABA assay / binding ELISA.
纯度(Purity)
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系(QMS)
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数据展示
电泳(SDS-PAGE)
Biotinylated Mouse SIRP alpha Protein, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Biotinylated Mouse SIRP alpha Protein, Fc,Avitag (Cat. No. SIA-M82F7) is more than 90% and the molecular weight of this protein is around 175-205 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA
Immobilized Biotinylated Mouse SIRP alpha Protein, Fc,Avitag (Cat. No. SIA-M82F7) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mouse CD47, His Tag (Cat. No. CD7-M522b) with a linear range of 0.078-2.5 μg/mL (QC tested).
Protocol
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