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PE-Labeled Human Siglec-10 Protein, His Tag (recommended for neutralizing assay) (Site-specific conjugation)

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SI0-HP2E5-50tests
¥3150.00
合计0件 产品金额¥ 0

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  • 分子别名(Synonym)

    SIGLEC10,MGC126774,PRO940,Siglec10,SLG2

  • 表达区间及表达系统(Source)

    PE-Labeled Human Siglec-10, His Tag (SI0-HP2E5) is produced via site-specific conjugation of PE to Human Siglec-10, His Tag under optimal conditions with a proprietary technology with a proprietary technology. Human Siglec-10, His Tag is expressed from human 293 cells (HEK293). It contains AA Met 17 - Thr 546 (Accession # Q96LC7-1).

    Predicted N-terminus: Met 17

    Request for sequence

  • 蛋白结构(Molecular Characterization)

    Siglec-10 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 61.8 kDa.

  • 偶联(Conjugate)

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • 应用说明(Application)

    Flow Cytometry (Neutralizing assay), Please note that this product is NOT compatible to streptavidin detection system.

    Designed and validated to use together with CD24 overexpressing HEK293 cells.

    Not suitable to use together with overexpressing CHO cells for high non-specific background from proteins of high sialylation.

    Not recommended and validated to use together with tumor cells.

  • 制剂(Formulation)

    Supplied as 0.2 μm filtered solution in PBS with Arginine, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 运输(Shipping)

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • 存储(Storage)

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 活性(Bioactivity)-FACS

     Siglec-10 FACS

    2e5 of 293 cells overexpressing human CD24 were stained with 100μL of 1:10 dilution of PE-Labeled Human Siglec-10 Protein, His Tag(recommended for neutralizing assay)(Cat. No. SI0-HP2E5)and negative control protein respectively , washed and analyzed with FACS (QC tested).

    Protocol

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背景介绍

The siglecs (sialic acid-binding Ig-like lectins) are a distinct subset of the Ig superfamily with adhesion-molecule-like structure. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products.

前沿进展

 
药物研发进展
  • 英文全称:

    Sialic acid-binding Ig-like lectin 10

  • 中文全称:

    唾液酸结合免疫球蛋白样凝集素-10

  • 种类:

  • 上市药物数量:

    0 详情

  • 临床药物数量:

    4 详情

  • 最高研发阶段:

    临床三期

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